1,10-Phenanthroline, Monohydrate

Catalog #

27713

$205 *

Size: 0.5 ml

Qty

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Description

1,10-phenanthroline (o-phenanthroline) is a chelator with high affinity for divalent metal ions. When complexed with copper, it possesses nuclease activity that has been used to study DNA-protein interactions. While not specific for DUBs, it has been shown to be a potent inhibitor of JAMM-type isopeptidases through chelation of the active site Zn²⁺ ion. As such, o-phenanthroline is widely used when preparing cell lysates for enrichment of proteins bearing K63-linked polyubiquitin chains which are especially sensitive to DUBs in the JAMM family.

This product has been cited 2 times.

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Synonyms

o–phenanthroline

Product Info

Storage and Usage

Citations2

Purity

≥99%

Supplied As

0.5M solution in anhydrous DMSO (100X)

Format

DMSO solution

Formula

C12H8N2 · H2O

198.2 Da

Solubility

Soluble in ethanol or methanol (200 mM). Weakly soluble in aqueous solutions.

Biological Activity

Metalloprotease inhibitor; chelates iron, zinc and other divalent metals. Effective concentration 1-10 mM.

CAS Registry #

5144-89-8

References

1. Cooper, E. M., Cutcliffe, C., Kristiansen, T. Z., Pandey, A., Pickart, C. M., and Cohen, R. E. (2009) K63-specific deubiquitination by two JAMM/MPN+ complexes: BRISC-associated Brcc36 and proteasomal Poh1. EMBO J. 28:621-631.
2. Sigman, D.S., et al., Nuclease activity of 1,10-phenanthroline-copper in study of protein DNA interactions. Methods Enzymol., 208:414-433 (1991).

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1,10-Phenanthroline, Monohydrate

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