Ubiquitin AMC

Catalog #
81150
$380 *
Size: 50 µg
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Description

Ubiquitin-AMC is a fluorogenic substrate for ubiquitin hydrolases based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC). Upon incubation with a protease recognizing Ubiquitin, such as USP2 or UCHL3, AMC is released and the increase in fluorescence can be measured using Ex380 nm and Em460 nm wavelengths. This protein contains no extraneous tags.

This product has been cited 1 time.

Synonyms
Ub-AMC
Product Info
Storage and Usage
Citations1
Species
Human
Purity
≥95% by HPLC.
Format
Lyophilized solid
MW
8722 Da
Solubility
Soluble in DMSO or aqueous buffers (> 1 mg/ml)
Biological Activity
Typical enzyme concentrations for UCH-L3 are 10-100 pM and for Isopeptidase-T are 10-100 nM.
Genbank #
P62987 (ubiquitin)
UniProt #
P09936
Background
Ubiquitin-AMC is prepared via the conjugation of the C-terminus of mature, Ubiquitin with 7-amino-4-methylcoumarin (AMC). This conjugation quenches the intrinsic fluorescence of AMC. The high purity of BPS's Ubiquitin-AMC compared to that of other commercial vendors leads to a greater signal:background.
References
1. Dang, L.C., et al. Kinetic and mechanistic studies on the hydrolysis of ubiquitin C-terminal 7-amido-4-methylcoumarin by deubiquitinating enzymes. Biochemistry, 37, 1868-1879 (1998).
2. Mason, D.E., et al. Substrate profiling of deubiquitin hydrolases with a positional scanning library and mass spectrometry. Biochemistry, 43, 6535-44 (2004).