UbcH7, His-tag (Sf9-derived) Recombinant

Catalog #

80318

$290 *

Size: 50 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

UbcH7, also known as UBE2L3 (GenBank Accession No. NM_003347), a.a. 1-154 (end), with C-terminal His-tag, MW = 18.7 kDa, expressed Sf9 cells via a baculovirus expression system.

●

Synonyms

UbcH7, UBE2L3, ubiquitin-conjugating enzyme E2L3

●

Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥71%

Format

Aqueous buffer solution

Formulation

50 mM Tris, pH 8.0, 138 mM NaCl, 2.7 mM KCl, 0.05% Tween-20, 50% glycerol and 1 mM DTT

18 kDa

Amino Acids

1–154

Genbank #

NM_003347

UniProt #

P68036

Tag(s)

C-terminal His-tag

Storage/Stability

>6 months at -80°C.

Assay Conditions

50 μl reaction mix (4 mM ATP, 5 mM MgCl2, 1 mM DTT, UBE1 (0.5 μg), UbcH7, Biotin-Ubiquitin (0.5 μg) and Smurf2 (1.5 μg) in 50 mM Tris, pH 7.4) for 30 min at 37°C. Add to Streptavidin-coated plates for 30 min at 37°C. Incubate with anti-Smurf2, then HRP-labeled secondary antibody, and chemiluminescent detection.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful in conjunction with E1 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ubiquitin Conjugating Enzyme (E2)

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

●

Datasheets/Protocols

80318_Lot120103.pdf

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

● General Protocols

● FAQs

● Contact Technical Service

●

UbcH7, His-tag (Sf9-derived) Recombinant

PRODUCTS

SERVICES

RESOURCES

COMPANY