UBA6 (UBE1L2), FLAG-tag Recombinant

Catalog #

80303

$290 *

Size: 100 µg

Qty

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Description

Human UBA6, also known as UBE1L2 (GenBank Accession No. NM_018227), a.a. 2-end, with N-terminal FLAG-tag, MW=118 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

UBA6, UBE1L2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

UBA6 inhibition curve using a TR-FRET assay (#82216). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 µg/ml FLAG peptide, 20% glycerol, and 3 mM DTT.

118 kDa

Amino Acids

2–end

Genbank #

NM_018227

UniProt #

A0AVT1

Tag(s)

N-terminal FLAG-tag

References

1. Groettrup M. et al., Trends Biochem. Sci. 33 (5), 230-237 (2008).
2. Pelzer C. et al., J. Biol. Chem. 282 (32), 23010-23014 (2007).

Storage/Stability

>6 months at -80°C.

Assay Conditions

Reaction mixture containing UBA6 (UBE1L2) as a positive control, Ubc5a (500 ng), and FLAG- Smurf2 (500 ng) was incubated at 37°C for 30 minutes with biotinylated ubiquitin. The reaction mix was incubated with Anti-FLAG for 1 hour, then HRP secondary Ab for 30 min, followed by chemiluminescent detection.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful in conjunction with E2 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ubiquitin Activating Enzyme (E1)

Regulatory Status

Research Use Only

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Datasheets/Protocols

80303_Lot120824.pdf 80303_Lot120523.pdf 80303_Lot100512.pdf

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UBA6 (UBE1L2), FLAG-tag Recombinant

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