Ultra-pure version of Benzonase. Nonspecific endonuclease, hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5’-phosphorylated oligonucleotides of 1-4 bases in length. MW = 27 kDa (homodimer). Expressed in E. coli. No detectable protease activity.
TurboNuclease can be used to • reduce viscosity of cell lysates • reduce back pressure of column loading • remove nucleic acid contamination from sample preparations • reduce nucleic acid contamination of Ni column purification • reduce smearing in SDS-PAGE when used with 10%SDS or Gel Loading Dye • make whole cell lysates • reduce or prevent clumping of concentrated cells and thawed cells • replace crude DNase I in many applications
250 units/μl in 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 5 mM MgCl2 and 50% glycerol
MW
27 kDa
Endotoxin Level
Total endotoxin level is <0.1EU/1,000 units of TurboNuclease as determined by the Endosafe® PTSTM LAL test system. No protease activity was detected.
Specific Activity
≥ 1.3 x 106 units/mg (> 3 x 106 Kunitz units/mg)
Unit Definition
One unit converts 1 OD260 of salmon sperm DNA into acid soluble nucleotides in 30 minutes at 37°C
Storage/Stability
>6 months at –20°C. Retains >80% activity after storage at RT for over 65 hours.
Assay Conditions
Reaction buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl2.
Instructions for Use
To reduce viscosity of cell lysate, 10-500 units of TurboNuclease can be used for each gram of cell paste. Generally, adding TurboNuclease to cell lysate at 25 U/ml is sufficient to reduce lysate viscosity. The efficiency of viscosity reduction may vary with buffers, cell types, and cell lysis methods used. Due to its high specific activity, the total amount TurboNuclease added is less than 0.1 μg/ml of lysate and will not complicate any downstream processes.
Applications
Ideal to digest nucleic acids and to reduce viscosity during protein purification and sample preparation.
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