RARγ Reporter Cellular Assay Pack

Catalog #

79324

$2,050 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The RARγ Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of retinoic acid receptor gamma (RARγ). The pack contains the RARγ Reporter (Luc)-HEK293 Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARγ (GenBank Accession No. P13631-1). This cell line is functionally validated for the response to the stimulation of all-trans retinoic acid, and the expression of RARγ is confirmed by Western blotting.

Additionally, the pack includes cell culture medium (Thaw Medium 6) that has been optimized for use with HEK293 cells. Thaw Medium 6 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required.

This product has been cited 2 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

RAR Gamma, Retinoic Acid Receptor Gamma, RARG

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Product Data Gallery

Product Info

Storage and Usage

Citations2

Materials Required But Not Supplied

• All-Trans Retinoic Acid (ATRA) (Sigma-Aldrich, #R2625): stock solution in DMSO

• 96-well tissue culture treated white clear-bottom assay plate (Corning, #3610)

• Luminometer

Format

Category Number	Component	Amount	Storage
60604	RARγ Reporter (Luc) - HEK293 Cell Line	2 vials*	-80°C	Avoid freeze/ thaw cycles!
60690-1	ONE-Step Luciferase Buffer (Component A)	10 ml	-20°C
60690-1	ONE-Step Luciferase Reagent Substrate, 100x (Component B)	100 µl	-20°C
60183	Thaw Medium 6	100 ml	+4°C

*Each vial contains ~2 X 10⁶ cells in 1 ml of 10% DMSO.

Background

Retinoic acid receptor (RAR) belongs to a family of nuclear receptors and has three subtypes, RARα, RARβ, and RARγ. RAR heterodimerizes with RXR (retinoid X receptor) and acts as a transcription factor that regulates the growth and differentiation of both normal and malignant cells. When RAR binds to its ligands, all-trans retinoic acid or 9-cis retinoic acid, RAR/ RXR heterodimer binds to retinoic acid response elements in the promoter region of target genes and recruits coactivator proteins, leading to transcription and expression of the downstream target genes.

References

1. Petkovich, M, et al. Nature (1987) 330(6147): 444-450.

2. Allenby, G, et al. Proc. Natl. Acad. Sci. USA (1993) 90(1): 30-34.

Application Reference(s):

1. Selective Agonists of Nuclear Retinoic Acid Receptor Gamma Inhibit Growth of HCS-2/8 Chondrosarcoma Cells (2019)
2. Understanding the Action of RARγ Agonists on Human Osteochondroma Explants (2020)

Storage/Stability

Stable at least 12 months from date of receipt, when stored as directed (-20°C).

Growth Media

Thaw Medium 6 (BPS Bioscience, #60183): DMEM supplemented with 10% FBS and 1% Penicillin/Streptomycin.

Complete Growth Medium: DMEM, 10% FBS, 1% Penicillin/Streptomycin, plus 400 µg/ml Geneticin (G418), 1 µg/ml Puromycin, and 100 µg/ml Hygromycin.

Instructions for Use

See data sheet insert for detailed protocols.

Applications

Monitor RARγ-regulated pathway activity
Screen agonists or antagonists of RARγ.

Shipping Temperature

-80°C

Notes

License Disclosure

Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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RARγ Reporter Cellular Assay Pack

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