The RARα Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of retinoic acid receptor alpha (RARα). The pack contains the RARα Reporter (Luc)-HEK293 Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARα (GenBank Accession No. NM_000964). This cell line is functionally validated for the response to the stimulation of all-trans retinoic acid, and the expression of RARα is confirmed by Western blotting.
Additionally, the pack includes cell culture medium (Thaw Medium 6) that has been optimized for use with HEK293 cells. Thaw Medium 6 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
*Each vial contains ~2 X 106 cells in 1 ml of 10% DMSO.
Background
Retinoic acid receptor (RAR) belongs to a family of nuclear receptors and has three subtypes, RARα, RARβ, and RARγ. RAR heterodimerizes with RXR (retinoid X receptor) and acts as a transcription factor that regulates the growth and differentiation of both normal and malignant cells. When RAR binds to its ligands, all-trans retinoic acid or 9-cis retinoic acid, RAR/ RXR heterodimer binds to retinoic acid response elements in the promoter region of target genes and recruits coactivator proteins, leading to transcription and expression of the downstream target genes.
References
1. Petkovich, M, et al. Nature (1987) 330(6147): 444-450.
2. Allenby, G, et al. Proc. Natl. Acad. Sci. USA (1993) 90(1): 30-34.
Storage/Stability
Stable at least 12 months from date of receipt, when stored as directed (-20°C).
Complete Growth Medium: DMEM, 10% FBS, 1% Penicillin/Streptomycin, plus 400 µg/ml of Geneticin (G418), 1 µg/ml of Puromycin, and 100 µg/ml Hygromycin.
Instructions for Use
See data sheet insert for detailed protocols.
Shipping Temperature
-80°C
Notes
License Disclosure
Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.
Warnings
Avoid freeze/thaw cycles.
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.