NF-κB Reporter Cellular Assay Pack (CHOK1)

Catalog #

79325

$1,245 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The NF-κB Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of the nuclear factor Kappa B (NF-κB) signal transduction pathways. The pack contains the NF-κB Reporter (Luc) - CHO-K1 Recombinant Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. This cell line is validated for the response to TNFalpha and to treatment with NF-κB inhibitor, evodiamine.

Additionally, the pack includes cell culture medium (Thaw Medium 3) that has been optimized for use with CHO-K1 cells. Thaw Medium 3 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

NF-κB Cell Line, NFkappaB Cell Line, Luciferase Reporter, NF-kB Cell Line, NFkB Cell Line, nfkb, nf-kb

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Product Data Gallery

Product Info

Storage and Usage

Citations

Materials Required But Not Supplied

• IL-1β (BPS Bioscience, #90168)
• Ham’s F-12 medium (Hyclone, # SH30526.01)
• TNFα, human (BPS Bioscience, #90244)
• TNFα, mouse (BPS Bioscience, #90246)
• IL-17A (BPS Bioscience, #91014)
• IFN-gamma (BPS Bioscience, #90162)
• Growth Medium 3D (BPS Bioscience, #79539)
• Phorbol 12-myristate 13-acetate (Sigma, #P1585)
• 96-well tissue culture treated white clear-bottom assay plate (Corning, #3610)
• Luminometer

Format

References

1. Delude, R.L., et.al. (1994) CD14-mediated Translocation of Nuclear Factor-kB Induced by Lipopolysaccharide Does Not Require Tyrosine Kinase Activity. J. Biol. Chem. 269: 22253

2. Railo, A., et.al. (2008) Wnt-11 signaling leads to down-regulation of the Wnt/beta-catenin, JNK/AP-1 and NF-kappaB pathways and promotes viability in the CHO-K1 cells. Exp Cell Res. 314: 2389-99

3. Murphy, S.H., et.al. (2011) Tumor suppressor protein (p)53, is a regulator of NF-κB repression by the glucocorticoid receptor. Proc. Natl. Acad. Sci. USA 108: 17117-17122

Storage/Stability

Stable at least 12 months from date of receipt, when stored as directed (-20°C).

Growth Media

Thaw Medium 3 (BPS Bioscience, #60186): Ham’s F-12 medium supplemented with 10% FBS and 1% Penicillin/Streptomycin.

Growth Medium 3D (BPS Bioscience, #79539): Ham’s F-12 medium,10% FBS, 1% Penicillin/Streptomycin plus 1 mg/ml Geneticin (G418).

Instructions for Use

See data sheet insert for detailed protocols.

Shipping Temperature

-80°C

Notes

License Disclosure

Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact [email protected] for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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NF-κB Reporter Cellular Assay Pack (CHOK1)

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