TPH1 Inhibitor Screening Assay Kit

Catalog #
72056
$1,130 *
Size: 384 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The TPH1 Assay Kit is designed to measure TPH1 enzyme inhibition in a 384 reaction format. This fluorescence-based assay kit is especially suitable for high throughput screening applications. The procedure is quick, straightforward, and simple - just mix two solutions, incubate, add quenching solution, and measure the fluorescence.

Synonyms
tryptophan 5-hydroxylase, TRPH, activity assay kit
Product Info
Storage and Usage
Citations
Assay Kit Format
Fluorogenic
Format
Catalog
Number

Component

Amount

Storage
71192 TPH1, His-tag 2 x 50 µg -80°C

Avoid freeze/
thaw cycles!
  TPH Enzyme Solution 2 x 5 ml -80°C
  TPH Reaction Solution 2 x 6 ml -80°C
  TPH Quench Solution 3 x 1 ml -20°C
79961 Black, 384 Well Plate 1 Room Temp.  
UniProt #
P17752
Background
Tryptophan 5-hydroxylase 1 and 2 (TPH1 and TPH2) are enzymes that catalyze the monooxygenation of tryptophan to 5-hydroxytryptophan (5-HTP) which is subsequently converted to serotonin. By catalyzing the rate-limiting step in the biosynthesis of serotonin, these enzymes also play key roles in the signaling of other neurotransmitters downstream of serotonin synthesis, like melatonin. Additionally, TPH enzymes may play a role in immune regulation through tryptophan depletion.
References

1. Moran G. R., et al. J. Biol. Chem. 1998; 273:12259-12266.
2. Nowak E. C., et al., J. Exp. Med. 2012; 209:2127-2135.