TNKS2 Histone Ribosylation Assay Kit (Biotin-labeled NAD+)

Catalog #: 80578
Size: 96 reactions
TNKS2 catalyzes the NAD-dependent addition of poly(ADP-ribose) to the substrate proteins.
The TNKS2 Histone Ribosylation Assay Kit (Biotin-labeled NAD+) is designed to measure Tankyrase 2 (TNKS2) activity for screening and profiling applications. The key to the TNKS2 Histone Ribosylation Assay is the biotinylated NAD+ substrate. With this kit, only three simple steps are required for TNKS2 reactions. First, histone proteins are coated on a 96-well plate. Next, the biotinylated NAD+ substrate is incubated with an assay buffer that contains the TNKS2 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
Tankyrase2, TNKS2, poly (ADP-ribose) polymerase 5, poly(ADP-ribosyl)transferase, PARP5b, PARP5c
Supplied As:
The TNKS2 assay kit comes in a convenient 96-well format, with purified TNKS2 enzyme, histone mixture, and PARP assay buffer for 100 enzyme reactions.
DMSO >1%, strong acids or bases, ionic detergents, high salt


Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Brown, J.A., Marala, R.B. J. Pharmacol. Toxicol. Methods 2002 47:137-41.

Application Reference(s):
Disruption of Wnt/β-Catenin Signaling and Telomeric Shortening Are Inextricable Consequences of Tankyrase Inhibition in Human Cells (2015)

Avoid freeze/thaw cycles.
Scientific Category:
Product Type:
Assay Kit
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