TNKS2 Histone Ribosylation Assay Kit (Biotin-labeled NAD+) (Discontinued)

Catalog #

80578

$875 *

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Description

The TNKS2 Histone Ribosylation Assay Kit (Biotin-labeled NAD+) is designed to measure Tankyrase 2 (TNKS2) activity for screening and profiling applications. The key to the TNKS2 Histone Ribosylation Assay is the biotinylated NAD+ substrate. With this kit, only three simple steps are required for TNKS2 reactions. First, histone proteins are coated on a 96-well plate. Next, the biotinylated NAD+ substrate is incubated with an assay buffer that contains the TNKS2 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

This product has been cited 2 times.

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Synonyms

Tankyrase2, TNKS2, poly (ADP-ribose) polymerase 5, poly(ADP-ribosyl)transferase, PARP5b, PARP5c

Product Info

Storage and Usage

Citations2

Assay Kit Format

Chemiluminescent

Supplied As

The TNKS2 assay kit comes in a convenient 96-well format, with purified TNKS2 enzyme, histone mixture, and PARP assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

1x PBS buffer
PBST buffer (1x PBS, containing 0.05% Tween-20)
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Format

Catalog Number	Component	Amount	Storage
80515	TNKS2	2 μg	-80°C	Avoid multiple freeze/ thaw cycles!
52029	5x histone mixture	1 ml	-80°C
80601	10x Assay Mixture Containing Biotinylated Substrate	300 μl	-80°C
80602	10x PARP assay buffer	1 ml	-20°C
79743	Blocking buffer 3	25 ml	+4°C
80611	Streptavidin-HRP	100 μl	+4°C
79670	ELISA ECL substrate (2 components)	6 ml each	Room Temp
79837	96-well module plate	1	Room Temp.

UniProt #

Q9H2K2

Background

TNKS2 catalyzes the NAD-dependent addition of poly(ADP-ribose) to the substrate proteins.

References

Brown, J.A., Marala, R.B. J. Pharmacol. Toxicol. Methods 2002 47:137-41.

Storage/Stability

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80°C

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PARP

Regulatory Status

Research Use Only

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TNKS2 Histone Ribosylation Assay Kit (Biotin-labeled NAD+) (Discontinued)

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