TNKS2 Histone Ribosylation Assay Kit (Antibody Detection) (Discontinued)

Catalog #

80577

$2,055 *

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Description

The Tankyrase-2 Histone Ribosylation Assay Kit (Antibody Detection) is designed to measure Tankyrase-2 activity for screening and profiling applications. The key to the Tankyrase-2 Histone Ribosylation Assay Kit (Antibody Detection) is a highly specific antibody that recognizes poly (ADP-ribose) modified histone. With this kit, only three simple steps on a microtiter plate are required for Tankyrase-2 reaction. First, NAD+ is incubated with a sample containing assay buffer and enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

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Synonyms

Tankyrase2, TNKS2, poly (ADP-ribose) polymerase 5, poly(ADP-ribosyl)transferase, PARP5b, PARP5c

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Supplied As

Kit comes in a convenient format, with a 384-well plate precoated with histone mixture, the antibody against poly (ADP-ribose) modified histone, the secondary HRP-labeled antibody, NAD+, TNKS assay buffer, and purified TNKS2 enzyme for 400 enzyme reaction

Format

CatalogNumber	Reagent	Amount	Storage
80515	TNKS2	4 µg	-80°C	*(Avoid freeze/thaw cycles!)*
	5× histone mixture	2 ml	-80°C
	NAD+	500 µl	-80°C
	10x PARP Assay Buffer	2 ml	-20°C
52140K	Primary antibody 11	26 µl	-80°C
52130H	Secondary HRP-labeled antibody 1	20 µl	-80°C
79743	Blocking Buffer	160 ml	+4°C
79670	HRP chemiluminescent substrate (2 components)	12 ml each	Room Temp
	384-well plate	1 plate	+4°C

UniProt #

Q9H2K2

References

Dillon SC, Zhang X, Trievel RC, Cheng X. Genome Biology 2005; 6:227.

Storage/Stability

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80

Notes

This kit is also suitable for use with cell extracts, but nonspecific ribosylation activity may be detected.

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PARP

Regulatory Status

Research Use Only

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TNKS2 Histone Ribosylation Assay Kit (Antibody Detection) (Discontinued)

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