Tankyrase 1 (PARP5A), GST-Tag Recombinant

Catalog #

80504

$515 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human Tankyrase 1 (TNKS1), also known as PARP5A, encompassing amino acids 1001-1327(end). This construct contains a N-terminal GST-tag, followed by a thrombin cleavage site. This protein was affinity purified.

This product has been cited 5 times.

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Synonyms

TNKS1, poly (ADP-ribose) polymerase 5a, poly(ADP-ribosyl)transferase 5a, PARP5A, TRF1-interacting ankyrin-related ADP-ribose polymerase 1, TANK1, tanykyrase-1, TNKS-1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

TNKS1 Activity

TNKS1 (PARP5A) inhibition curve using a chemiluminescent assay (#78405-1). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations5

Species

Human

Construct

TNKS1 (GST-1001-1327(end))

Host Species/Expression System

Sf9

Purity

≥80%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, and 20% glycerol.

63 kDa

Amino Acids

1001–1327(end)

Genbank #

NM_003747

UniProt #

O95271

Tag(s)

N-terminal GST-tag

Background

Tankyrase 1, also known as PARP5A (poly(ADP-ribose) polymerase 5a), belongs to the poly-ADP-ribosyltransferase family. It is involved in Wnt signaling, where it acts as an activator by interacting and poly-ADP-ribosylate AXIN1 and AXIN2 (negative regulators of Wnt pathway). Tankyrase also mediates PARsylation of TERF1 (telomeric repeat binding factor 1), inhibiting its binding to telomeric DNA and regulating its telomere length. Inhibitors targeting tankyrase are thus attractive molecules in cancer therapy.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Enzyme reaction is conducted using the TNKS1 Histone Ribosylation Assay Kit (Biotin-labeled NAD+) (BPS Bioscience #80573) with TNKS1 titrated from 100 ng/rxn – 1.6 ng/rxn.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PARP

Regulatory Status

Research Use Only

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Datasheets/Protocols

80504 Lot 210316-2

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Tankyrase 1 (PARP5A), GST-Tag Recombinant

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