Tankyrase 1 (PARP5A), GST-tag

Catalog #: 80504
Size: 10 µg
$450
Species: Human
Host Species: Sf9 insect cells
MW: 62.5 kDa
Genbank #: NM_003747
Tag(s): N-terminal GST-tag
a.a: 1001–1327(end)
Description:
TNKS1 (TRF1-interacting ankyrin-related ADP-ribose polymerase 1), also known as PARP5A, TANK1, or tanykyrase-1 (GenBank Accession No. NM_003747), a.a. 1001-1327(end), with N-terminal GST-tag, MW=62.5 kDa, expressed in Sf9 cells via a baculovirus expression system.
UniProt
O95271
Synonym(s):
TNKS1, poly (ADP-ribose) polymerase 5a, poly(ADP-ribosyl)transferase 5a, PARP5A, TRF1-interacting ankyrin-related ADP-ribose polymerase 1, TANK1, tanykyrase-1
Unit Definition:
One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.
Assay Conditions:
Enzyme reaction is conducted using BPS's TNKS1 Histone Ribosylation Assay Kit (Biotin-labeled NAD+), Catalog # 80573.
Formulation:
40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Chang S. (2010). Aging (Albany NY). 2(10):639-42.
2. Karner, C.M. et al. (2010). Dev Dyn. 239(7):2014-23.
3. McCabe, N, et al. (2009). Oncogene. 28(11):1465-70.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PARP
Product Type:
Recombinant Protein
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Product Datasheets

80504_Lot131030_1.pdf