TNKS1 Histone Ribosylation Assay Kit (Biotin-labeled NAD+) (Discontinued)

Catalog #
80573
$620 *
Catalog number 80573, TNKS1 Histone Ribosylation Assay Kit (Biotin-labeled NAD+) (Discontinued), has been discontinued. Please find our suggested alternative here.
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Description

The TNKS1 Histone Ribosylation Assay Kit (Biotin-labeled NAD+) is designed to measure Tankyrase 1 (TNKS1) activity for screening and profiling applications. The key to the TNKS1 Histone Ribosylation Assay (Biotin-labeled NAD+) is the biotinylated substrate. With this kit, only three simple steps are required for TNKS1 reactions. First, histone proteins are coated on a 96-well plate. Next, the biotinylated TNKS1 substrate is incubated with an assay buffer that contains the TNKS1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

This product has been cited 4 times.

Synonyms
Tankyrase1, TNKS1, poly (ADP-ribose) polymerase 5a, poly(ADP-ribosyl)transferase 5a, PARP5a
Product Info
Storage and Usage
Citations4
Assay Kit Format
Chemiluminescent
Supplied As
The TNKS1 assay kit comes in a convenient 96-well format, with purified TNKS1 enzyme, histone mixture, and PARP assay buffer for 100 enzyme reactions.
Materials Required But Not Supplied

1x PBS buffer
PBST buffer (1x PBS, containing 0.05% Tween-20)
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Format
Catalog Number Component Amount Storage
80504 TNKS1 5 μg -80°C
52029 5x histone mixture 1 ml -80°C
80601 10x Assay Mixture Containing Biotinylated Substrate 300 μl -80°C
80602 10x PARP assay buffer 1 ml -20°C
79743 Blocking buffer 3 25 ml +4°C
80611 Streptavidin-HRP 100 μl +4°C
79670 ELISA ECL substrate (2 components) 6 ml each Room
Temp
79837 96-well module plate 1 Room Temp.
UniProt #
O95271
Background
TNKS1 catalyzes the NAD-dependent addition of poly(ADP-ribose) to the substrate proteins.
References

Brown, J.A., Marala, R.B. J. Pharmacol. Toxicol. Methods 2002 47:137-41.