TNKS1 Histone Ribosylation Assay Kit (Biotin-labeled NAD+) (Discontinued)
The TNKS1 Histone Ribosylation Assay Kit (Biotin-labeled NAD+) is designed to measure Tankyrase 1 (TNKS1) activity for screening and profiling applications. The key to the TNKS1 Histone Ribosylation Assay (Biotin-labeled NAD+) is the biotinylated substrate. With this kit, only three simple steps are required for TNKS1 reactions. First, histone proteins are coated on a 96-well plate. Next, the biotinylated TNKS1 substrate is incubated with an assay buffer that contains the TNKS1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
This product has been cited 4 times.
1x PBS buffer
PBST buffer (1x PBS, containing 0.05% Tween-20)
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform
Catalog Number | Component | Amount | Storage |
80504 | TNKS1 | 5 μg | -80°C |
52029 | 5x histone mixture | 1 ml | -80°C |
80601 | 10x Assay Mixture Containing Biotinylated Substrate | 300 μl | -80°C |
80602 | 10x PARP assay buffer | 1 ml | -20°C |
79743 | Blocking buffer 3 | 25 ml | +4°C |
80611 | Streptavidin-HRP | 100 μl | +4°C |
79670 | ELISA ECL substrate (2 components) | 6 ml each | Room Temp |
79837 | 96-well module plate | 1 | Room Temp. |
Brown, J.A., Marala, R.B. J. Pharmacol. Toxicol. Methods 2002 47:137-41.