TNKS1 Histone Ribosylation Assay Kit (Antibody Detection) (Discontinued)

Catalog #
80575
$2,055 *
Catalog number 80575, TNKS1 Histone Ribosylation Assay Kit (Antibody Detection) (Discontinued), has been discontinued. Please find our suggested alternative here.
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Description

The Tankyrase-1 Histone Ribosylation Assay Kit (Antibody Detection) is designed to measure Tankyrase-1 activity for screening and profiling applications. The key to the Tankyrase-1 Histone Ribosylation Assay Kit (Antibody Detection) is a highly specific antibody that recognizes poly (ADP-ribose)-modified histone. With this kit, only three simple steps on a microtiter plate are required for Tankyrase-1 reaction. First, NAD+ is incubated with a sample containing assay buffer and enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Synonyms
Tankyrase1, TNKS1, poly (ADP-ribose) polymerase 5a, poly(ADP-ribosyl)transferase 5a, PARP5a
Product Info
Storage and Usage
Citations
Assay Kit Format
Chemiluminescent
Supplied As
Kit comes in a convenient format, with a 384-well plate precoated with histone mixture, antibody against poly (ADP-ribose) modified histone, the secondary HRP-labeled antibody, NAD+, TNKS assay buffer, and purified TNKS1 enzyme for 384 enzyme reactions.
Format

Catalog
Number


Reagent


Amount


Storage

80504

TNKS1, GST-tag

4 µg

-80°C







(Avoid freeze/
thaw cycles!)

 

NAD+

500 µl

-80°C

 

10x PARP Assay Buffer

2 ml

-20°C

52140K

Primary antibody 11

26 µl

-80°C

52130H

Secondary HRP-labeled antibody 1

20 µl

-80°C

79743

Blocking Buffer

160 ml

+4°C

79670

HRP chemiluminescent substrate (2 components)

12 ml each

Room
Temp

 

384-well plate pre-coated with histone mixture

 

1 plate

 

+4°C

UniProt #
O95271
References
Dillon SC, Zhang X, Trievel RC, Cheng X. Genome Biology 2005; 6:227.