TNKS1 Histone Ribosylation Assay Kit (Antibody Detection)

Catalog #: 80574
Size: 96 reactions
$1,025
Description:
The Tankyrase-1 Histone Ribosylation Assay Kit (Antibody Detection) is designed to measure Tankyrase-1 activity for screening and profiling applications. The key to the Tankyrase-1 Histone Ribosylation Assay Kit (Antibody Detection) is a highly specific antibody that recognizes poly (ADP-ribose)-modified histone. After coating the plate with the histone mixture, only three simple steps are required. First, NAD+ is incubated with a sample containing assay buffer and enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
Synonym(s):
Tankyrase1, TNKS1, poly (ADP-ribose) polymerase 5a, poly(ADP-ribosyl)transferase 5a, PARP5a
Supplied As:
Kit comes in a convenient format, with 8-well strips, histone mixture substrate, antibody against poly (ADP-ribose) modified histone, the secondary HRP-labeled antibody, NAD+, TNKS assay buffer, and purified TNKS1 enzyme for 100 enzyme reactions.
Contraindications:
DMSO >1%, strong acids or bases, ionic detergents, high salt
Format:
Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):
Dillon SC, Zhang X, Trievel RC, Cheng X. Genome Biology 2005; 6:227.
Notes:
This kit is also suitable for use with cell extracts, but nonspecific ribosylation activity may be detected.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PARP
Product Type:
Assay Kit
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Product Datasheets

80574.pdf