TIGIT, Fc Fusion, Avi-Tag, Biotin-Labeled (Human) HiP™ Recombinant

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Catalog #

71251

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Description

Recombinant human TIGIT (T cell immunoreceptor with Ig and ITIM domains), encompassing amino acids 22-141. The C-terminal end of the protein is fused with the Fc fragment of a human IgG1 followed by an Avi-Tag™. The recombinant protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified. HiP™ indicates a high purity protein (≥90% pure) and less than 10% aggregation as measured by gel filtration.

This product has been cited 2 times.

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Synonyms

T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9 (VSIG9), and V-set and transmembrane domaincontaining protein 3 (VSTM3), WUCAM

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

TIGIT [Biotinylated]: CD155 Interaction

Biotin-Avidin Pulldown

Gel Filtration Trace

TIGIT:CD112 inhibition curve using an AlphaLISA assay (#72030). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations2

Species

Human

Construct

TIGIT (22-141-Fc(IgG1)-Avi)-(Biotin)

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

42 kDa + glycans

Amino Acids

22-141

Glycosylation

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Aggregation

<10%

Genbank #

NM_173799

UniProt #

Q495A1

Tag(s)

C-terminal Fc fusion (IgG1), AviTag™

Label

This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation confirmed to be ≥90%.

For more information on enzymatic biotinylation, please see our Tech Note.

References

1. Yu, X., et al., Nat. Immunol. 2009; 10(1): 48-57.
2. Stanietsky, N., et al., Proc. Natl. Acad. Sci. (USA). 2009; 106(42): 17858-17863.

Storage/Stability

At least 6 months at -80°C

Assay Conditions

Assay was performed according to the protocol of the TIGIT:CD155 Homogeneous Assay Kit (BPS Bioscience #72029) with TIGIT titrated from 16 ng/rxn to 0 ng/rxn.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Datasheets/Protocols

71251 Lot 211014

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TIGIT, Fc Fusion, Avi-Tag, Biotin-Labeled (Human) HiP™ Recombinant

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