TIGIT:CD155 Homogeneous Assay Kit

Catalog #: 72029
Size: 384 reactions
Human T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is a receptor that is expressed on the surface of human T cells and NK cells that binds to CD155 and CD112 on the surface of dendritic cells. Binding of TIGIT with CD155 or CD112 results in inhibition of T cell and NK cell activation. Antibodies and other agents that inhibit this signaling pathway have been shown to increase the immune response, especially in the case of certain cancers.
The TIGIT:CD155 Homogeneous Assay Kit is designed to measure the inhibition of TIGIT binding to CD155. The TIGIT:CD155 Homogeneous Assay Kit comes in a convenient AlphaLISA® format with purified biotinylated TIGIT, His-tagged CD155, and assay buffer to perform a total of 384 reactions. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing TIGIT and an inhibitor of choice is incubated with the CD155 for 60 minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, VSIG9, V-set and transmembrane domain-containing protein 3, VSTM3, PVR, NECL-5, CD155-His, AlphaLISA, Alphascreen, Alpha Lisa, Alpha screen
Only limited amounts of DMSO can be included, as it has been shown to disrupt TIGIT:CD155 interaction. Avoid green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.

Instructions for use:
See assay datasheet for detailed protocol.
Storage / Stability:
At least one year from date of receipt when stored as directed.
Useful for screening for inhibitors of TIGIT binding to CD155

1. Yu, X., et al., Nat. Immunol. 2009; 10(1): 48-57.
2. Stanietsky, N., et al., Proc. Natl. Acad. Sci. 2009; 106(42): 17858-17863.

AlphaScreen® and AlphaLISA® are registered trademarks of PerkinElmer, Inc.
Avoid freeze/thaw cycles.
Scientific Category:
Immunotherapy/Cell Surface Receptor
Product Type:
Assay Kit
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