TIGIT:CD155 Homogeneous Assay Kit

Catalog #
72029
$495 *
Size: 384 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The TIGIT:CD155 Homogeneous Assay Kit is designed to measure the inhibition of TIGIT binding to CD155. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing TIGIT and an inhibitor of choice is incubated with the CD155 for 60 minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.

Synonyms
T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, VSIG9, V-set and transmembrane domain-containing protein 3, VSTM3, PVR, NECL-5, CD155-His
Product Info
Storage and Usage
Citations
Assay Kit Format
AlphaLISA®
Supplied As
The TIGIT:CD155 Homogeneous Assay Kit comes in a convenient AlphaLISA® format with purified biotinylated TIGIT, His-tagged CD155, and assay buffer to perform a total of 384 reactions.
Materials Required But Not Supplied

AlphaLISA Ni Chelate Acceptor beads, 5 mg/ml (PerkinElmer #AL108C)
AlphaScreen Streptavidin-conjugated Donor beads, 5 mg/ml (PerkinElmer #6760002S)
Optiplate-384 (PerkinElmer #6007290)
AlphaScreen microplate reader
Adjustable micropipettor and sterile tips

Format
Catalog
Number

Component

Amount

Storage
71251 TIGIT-Fc-biotin 2x 3 µg -80°C
Avoid freeze/

thaw cycles!
71181 CD155-His 2x 5 µg -80°C
79311 3x Immuno Buffer 1 4 ml -20°C
UniProt #
TIGIT: Q495A1; CD155: P15151
Background
Human T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is a receptor that is expressed on the surface of human T cells and NK cells that binds to CD155 and CD112 on the surface of dendritic cells. Binding of TIGIT with CD155 or CD112 results in inhibition of T cell and NK cell activation. Antibodies and other agents that inhibit this signaling pathway have been shown to increase the immune response, especially in the case of certain cancers.
References

1. Yu, X., et al., Nat. Immunol. 2009; 10(1): 48-57.
2. Stanietsky, N., et al., Proc. Natl. Acad. Sci. 2009; 106(42): 17858-17863.