TGFβ/Activin A-Responsive Luciferase Reporter HEK293 Cell Line

Catalog #

60653

$3,750 *

Size: 2 vials

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

The TGFβ/Activin A-Responsive Reporter HEK293 Cell Line is a HEK293 cell line expressing the Firefly luciferase reporter under the control of SMAD-responsive elements (SMAD binding elements, SBE). This cell line monitors the activity of the TGFβ (transforming growth factor beta)/SMAD signaling pathway.

This cell line has been validated by activation in response to human TGFβ1 and to other cytokines of the TGFβ1 superfamily such as Activin A, GDF-8/Myostatin, and GDF-11/BMP-11. Luciferase activity induced by human TGFβ1 was decreased by SB525334, an inhibitor of the TGFβ1-receptor. Luciferase activity induced by Activin A, GDF-8/Myostatin, and GDF-11/BMP-11 was decreased by Bimagrumab, an antibody of the ActRII. Activin A induced luciferase activity was also decreased by Activin Blocker, an ActRIIA-Fc fusion protein.

Figure 1: illustration of mechanism of action of the TGFβ/Activin A-Responsive Reporter HEK293 Cell Line.

This product has been cited 13 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

●

Synonyms

SBE cell line, TGFbeta reporter, SMAD reporter, luciferase reporter, TGFβ, TGF-β, activin, TGFb-1, TGFb1

●

Product Data Gallery

Reporter activation in the TGFβ/Activin A-Responsive Luciferase Reporter HEK293 Cell Line by the agonist TGFβ1.

Reporter activation in the TGFβ/Activin A-Responsive Luciferase Reporter HEK293 Cell Line by the agonist Activin A.

Reporter activation in the TGFβ/Activin A-Responsive Luciferase Reporter HEK293 Cell Line by the agonist GDF-8/Myostatin

Reporter activation in the TGFβ/Activin A-Responsive Luciferase Reporter HEK293 Cell Line by the agonist GDF-11/BMP-11.

Inhibition of TGFβ1-induced reporter activity by SB525334 in the TGFβ/Activin A-Responsive Luciferase Reporter HEK293 Cell Line.

Product Info

Storage and Usage

Citations13

Host Cell Line

HEK293, Human Embryonic Kidney, epithelial-like cells, adherent

Species

Human

Supplied As

Each vial contains >1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 1	BPS Bioscience #60187
Growth Medium 1B	BPS Bioscience #79531

Materials Used in Cellular Assay

Name	Ordering Information
Assay Medium 1B	BPS Bioscience #79617
Human TGFβ1	BPS Bioscience #90900
Recombinant Human/Murine/Rat Activin A	Pepro Tech #120-14E
Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein	R&D Systems #788-G8
Recombinant Human/Mouse/Rat GDF-11/BMP-11 Protein	R&D Systems #1958-GD
Inhibitor SB525334	Selleckchem #356559-20-1
Bimagrumab	MedChemExpress #HY-P99355
Activin Blocker	BPS Bioscience #102121
Clear-bottom, white 96-well tissue culture-treated plate	Corning #3610
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
Luminometer

UniProt #

P01137, P61812, P10600, Q15796, P84022, Q13485

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

The TGFβ signaling pathway participates in diverse cellular processes such as growth and differentiation, cell cycle arrest, and immune regulation. TGFβ signaling has been linked to heart disease, cancer, Alzheimer’s disease, among others. TGFβ proteins bind to receptors on the cell surface, initiating a signaling cascade that leads to phosphorylation and activation of the signaling proteins SMAD2 (mothers against decapentaplegic homolog 2) and SMAD3, which then form a complex with SMAD4. The SMAD complex gets translocated to the nucleus and binds to the SMAD binding element (SBE) in the promoter of target genes, leading to transcription and expression of TGFβ/ SMAD responsive genes. The understanding and regulation of this pathway can provide essential clues for the development of therapeutical approaches for cancer treatment.

References

Moustakas A, et al., 2001 J. Cell Science. 114: 4359-69

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin.

Growth Medium 1B (BPS Bioscience #79531): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 400 µg/ml of Geneticin.

Media Required for Functional Cellular Assay
Assay Medium 1B (BPS Bioscience #79617): MEM Medium, 0.5% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin

Applications

Monitor activation of the TGFβ signaling pathway.
Screen for compounds that regulate the TGFβ/SMAD signaling pathway.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only