TET2, FLAG-Tag, His-Tag (catalytic domain) Recombinant

Catalog #

50162

$485 *

Size: 50 µg

Qty

Bulk/Custom

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Synonyms

Ten-eleven translocation 2 protein, methylcytosine dioxygenase TET2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

TET2 inhibition curve using a chemiluminescent assay (#50652). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 cells

Purity

80%

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT, 140 mM imidazole.

100 kDa

Amino Acids

1129-2002(end)

Genbank #

NM_001127208

UniProt #

Q6N021

Tag(s)

N-terminal FLAG-tag, C terminal His tag

References

1. Myunggon, K., et al., Nature. 2013;497: 122-126.

2. Shinsuke, I, et al., Nature. 2010; 466: 1129-1133.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay done according to TET2 Chemiluminescent Assay Kit (BPS Bioscience #50652) with TET2, FLAG-Tag, His-Tag (catalytic domain) titrated from 800 ng/rxn to 0 ng/rxn.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

dry ice

Warnings

Avoid freeze/thaw cycles.

Scientific Category

DNA Demethylase

Regulatory Status

Research Use Only