TET2 Chemiluminescent Assay Kit

Catalog #

50652

$760 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The TET2 Chemiluminescent Assay Kit is designed to measure TET2 activity for screening and profiling applications. TET2 belongs to the Ten Eleven Translocation (TET) family proteins that catalyze 5-methylcytosine oxidation and generate 5-methylcytosine derivatives, including 5-hydroxymethylcytosine. TET2 is one of the most frequently mutated genes in myeloid malignancies.

The key to the TET2 Chemiluminescent Assay Kit is a highly specific antibody that recognizes hydroxymethylated substrate. With this kit, only three simple steps on a microtiter plate are required for detection of TET2 activity. First, TET2 enzyme is incubated with the methylated substrate for two hours. Then the primary antibody is added. Next, the plate is treated with an HRP-labeled secondary antibody. Finally, the HRP substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against TET2? Check out our Demethylase Screening Services.

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Synonyms

Ten-eleven translocation 2 protein, methylcytosine dioxygenase TET2

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Supplied As

The TET2 Chemiluminescent Assay Kit comes in a convenient format; a 96-well strip plate precoated with methylated DNA substrate, primary antibody, a secondary HRP-labeled antibody, TET2 assay buffer, and purified TET2 for 100 enzyme reactions.

Format

Catalog Number	Component	Amount	Storage
50162	TET2	40 µg	-80°C	Avoid multiple freeze/ thaw cycles!
52140Z2	Primary antibody 27	100 µl	-80°C
52131H	Secondary HRP-labeled antibody 2	10 µl	-80°C
79832	4x TET2 assay buffer 1	3 ml	-80°C
52100	Blocking buffer 4	50 ml	+4°C
79670	ELISA ECL Substrate A (transparent bottle)	6 ml	Room Temp.
79670	ELISA ECL Substrate B (brown bottle)	6 ml	Room Temp.
	8-well strip plate module precoated with DNA substrate	1	+4°C

UniProt #

Q6N021

References

Li Tan, Yujiang Geno Shi. Development 2012; 139(11): 1895-1902.

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Shipping Temperature

dry ice

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

DNA Demethylase

Regulatory Status

Research Use Only