TET1 Chemiluminescent Assay Kit

Catalog #

50651

$725 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The TET1 Chemiluminescent Assay Kit is designed to measure TET1 activity for screening and profiling applications. TET1 belongs to the Ten Eleven Translocation (TET) family proteins that catalyze 5-methylcytosine oxidation and generate 5-methylcytosine derivatives, including 5-hydroxymethylcytosine. TET1 may repress gene transcription in a catalytic activity-independent manner.

The key to the TET1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes hydroxymethylated substrate. With this kit, only three simple steps on a microtiter plate are required for detection of TET1 activity. First, the TET1 enzyme is incubated with the methylated substrate for two hours. Then the primary antibody is added. Next, the plate is treated with an HRP-labeled secondary antibody. Finally, the HRP substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against TET1? Check out our Demethylase Screening Services.

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Synonyms

Ten-eleven translocation 1 protein (TET1), methylcytosine dioxygenase TET1, Leukemiaassociated protein with a CXXC domain, CXXC-type zinc finger protein 6

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Supplied As

The TET1 Chemiluminescent Assay Kit comes in a convenient format, with a 96-well strip plate precoated with methylated DNA substrate, primary antibody, a secondary HRP-labeled antibody, TET1 assay buffer, and purified TET1 for 100 enzyme reactions.

Materials Required But Not Supplied

TBST buffer (1 x TBS, pH 8.0, containing 0.05% Tween-20)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Format

Catalog #	Component	Amount	Storage
50161	TET1, FLAG-tag	10 µg	-80°C	*Avoid freeze/ thaw cycles!*
52140Z2	Primary antibody 27	100 µl	-80°C
52131H	Secondary HRP-labeled antibody 2	10 µl	-80°C
52132	4x TET1 assay buffer 1	3 ml	-80°C
52100	Blocking buffer 4	50 ml	+4°C
79670	ELISA ECL substrate A (transparent bottle)	6 ml	Room Temp
79670	ELISA ECL substrate B (brown bottle)	6 ml	Room Temp
	8-well strip plate module precoated with DNA substrate	1	+4°C

UniProt #

Q8NFU7

References

Li Tan, Yujiang Geno Shi. Development 2012; 139(11): 1895-1902.

Application Reference(s):

Atm deficiency in the DNA polymerase β null cerebellum results in cerebellar ataxia and Itpr1 reduction associated with alteration of cytosine methylation (2020)

Storage/Stability

6 months from date of receipt when stored as directed.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and HTS applications.

Shipping Temperature

dry ice

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

DNA Demethylase

Regulatory Status

Research Use Only