TDO Cellular Activity QuickDetect™ Supplements
Catalog #
62002-1
$215
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Description
The TDO Cellular Activity QuickDetectTM Supplements are designed to complement the hTDO-HEK293 Recombinant Cell Line (BPS Cat. #60534) or other TDO-expressing cell lines. This kit contains the cell culture medium supplements necessary for activation of TDO and for the analysis and detection of tryptophan 2,3 dioxygenase (TDO)- catalyzed conversion of L-tryptophan (L-Trp) to Kynurenine (Kyn). The supplements and the detection reagents, when used as described, allow for indirect measurement of Kyn levels by analyzing absorption at 480 nm.
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Synonyms
tryptophan 2,3 dioxygenase, TDO2, TDO, TPH2, TRPO
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Background
L-Trp is an essential amino acid necessary for protein synthesis in mammalian
cells, and the L-Trp to Kyn pathway is firmly established as a key regulator of innate and
adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive
microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp
that have immunosuppressive functions. TDO is upregulated in many tumors, providing
cancer cells with an avenue for immune evasion.