TCF/LEF Reporter Kit (Wnt Signaling Pathway)

Catalog #: 60500
Size: 500 reactions
$550
Background:
The Wnt/β-catenin signaling pathway controls a large and diverse set of cell fate decisions in embryonic development, adult organ maintenance and disease. Wnt proteins bind to receptors on the cell surface, initiating a signaling cascade that leads to stabilization and nuclear translocation of β-catenin. β-catenin then binds to TCF/LEF transcription factors in the nucleus, leading to transcription and expression of Wnt-responsive genes.
Description:
The TCF/LEF Reporter kit is designed for monitoring the activity of Wnt / β-catenin signaling pathway in the cultured cells. The kit contains transfection-ready TCF/LEF luciferase reporter vector, which is a Wnt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized TCF/LEF responsive element located upstream of a minimal promoter. The TCF/LEF reporter is premixed with constitutively-expressing Renilla luciferase vector that serves as internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity.
Synonym(s):
WNT reporter, TCF reporter, LEF reporter, luciferase reporter , Cell based assay kit, cell-based assay, reporter assay kit
Supplied As:
Reporter: TCF/LEF luciferase reporter vector + constitutively-expressing Renilla luciferase vector
Negative Control Reporter: Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector
Concentration:

Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each)

Format:
assay kit
Instructions for use:
See data sheet for detailed transfection and assay protocols.
Storage / Stability:

Stable at least 12 months from date of receipt, when stored as directed (-20°C)

Application(s):
• Monitor Wnt /β-catenin signaling pathway activity.
• Screen for activators or inhibitors of the Wnt /β-catenin signaling pathway.
• Study effects of RNAi or gene overexpression on the activity of the Wnt /β-catenin pathway.
Reference(s):
1. Clevers H (2006) Wnt/beta-catenin signaling in development and disease. Cell 127(3):469-480.
2. Chen B et al. (2009) Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nature Chemical Biology 5(2):100-107.
Notes:
The vectors are suitable for transient transfection. They are NOT suitable for transformation and amplification in bacteria.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Cell Signaling Pathway, WNT
Product Type:
Reporter Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

60500.pdf