TCF/LEF Reporter – HEK293 Cell Line (Wnt Signaling Pathway, Lithium-Independent)

Catalog #: 60531
Size: 2 vials
The Wnt Signaling Pathway TCF/LEF Reporter (Luc) – HEK293 Cell Line (Lithium-Independent) is designed for monitoring the activity of the Wnt/b-catenin signaling pathway, without the requirement for external LiCl. The Wnt pathway controls a large and diverse set of cell fate decisions in embryonic development, adult organ maintenance and disease. Wnt proteins bind to receptors on the cell surface, initiating a signaling cascade that leads to stabilization and nuclear translocation of b-catenin. b-catenin then binds to TCF/LEF transcription factors in the nucleus, leading to transcription and expression of Wnt-responsive genes.
The Wnt Signaling Pathway TCF/LEF Reporter (Luc) – HEK293 Cell Line contains a firefly luciferase gene under the control of TCF/LEF responsive elements stably integrated into HEK293 cells. This cell line is activated by Wnt3a alone, without the need for Lithium Chloride to boost the signal. It is validated for response to the stimulation of mouse Wnt3a and to treatment with an inhibitor of the Wnt/b-catenin signaling pathway.
Materials Required But Not Provided:

• Mouse Wnt3a (R&D Systems 1324-WN)
• IWR-1-endo (Santa Cruz Biotechnology # sc-295215): inhibitor of Wnt pathway
• Assay medium: Thaw Medium 1 (BPS Cat. #60187)
Growth Medium 1B (BPS Cat. #79531)
• 96-well tissue culture treated white clear-bottom assay plate (Corning #3610)
• ONE-Step™ luciferase assay system (BPS Cat. #60690) or other luciferase reagents for measuring firefly luciferase activity
• Luminometer

Supplied As:
Each vial contains ~1.5 X 10^6 cells in 1 ml of 10% DMSO.
Mycoplasma Testing:
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Growth Media:

Thaw Medium 1 (BPS Cat. #60187): MEM medium (Hyclone #SH30024.01) supplemented with 10% FBS (Invitrogen #26140-079), 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone SV30010.01).

Growth Medium 1B (BPS Cat. #79531): Thaw Medium 1 plus 400 μg/ml of Geneticin (Life Technologies #11811031).

Aqueous solution containing DMSO.
Instructions for use:
See datasheet for detailed assay and culture instructions.
Storage / Stability:
Immediately upon receipt, store in liquid nitrogen.
• Monitor Wnt signaling pathway activity.

• Screen activators or inhibitors of Wnt/b-catenin signaling pathway.


1. Clevers, H. (2006). Wnt/beta-catenin signaling in development and disease. Cell 127(3):469-480.
2. Chen, B. et al. (2009) Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nature Chemical Biology 5(2):100-107.

Application References:
1. Activation of anti-oxidant Nrf2 signaling by enone analogues of curcumin (2017)
2. Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin/α-albumin (2016)


License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Scientific Category:
Cell Signaling Pathway, Wnt
Product Type:
Cell Line
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