TC-PTP(PTPN2), GST-Tag Recombinant

Catalog #

30013

$325 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Human recombinant TC-PTP, also known as PTPN2 (protein tyrosine phosphatase N2), Genbank accession No. BC016727, a.a. 2-315 (catalytic domain), with N-terminal GST tag, MW=62.3 kDa, expressed in an E. coli expression system.

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Synonyms

PTPN2, TC-PTP, PTPT, TCELLPTP, TCPTP

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and variable glutathione.

62.3 kDa

Amino Acids

2–315

Specific Activity

1,000 pmole/min/µg

Unit Definition

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Genbank #

BC016727

UniProt #

P17706

Tag(s)

N-terminal GST-tag

Background

Protein tyrosine phosphatase, non-receptor type 2 (PTPN2), is one of the most abundant mammalian tyrosine phosphatase. The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. By virtue of protein tyrosine phosphatase activity, PTPN2 is known to be a signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation involved in cell communication and signal transduction.

References

1. N.F. Zander et al., Biochemistry 1991, 30: 6964.
2. D.E. Cool et al., Proc. Natl. Acad. Sci. USA 86: 5257.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Enzyme reaction (50 µl) containing 20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1 mM pNPP and PTPase was monitored at 415 nm continuously. Specific activity was calculated based on ƐpNP- = 18,000 M-1cm-1

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

PTP/Non-rec.

Regulatory Status

Research Use Only