TAF1L (BD1+BD2), GST-tag

Catalog #: 31107
Size: 100 µg
$295
Species: Human
Host Species: E. coli
MW: 56.1 kDa
Genbank #: NM_153809
Tag(s): N-terminal GST-tag
a.a: 1398-1649
Description:
Human TAF1 RNA polymerase II, TAT box binding protein (TBP)-associated factor, also known as TAF1L, GenBank Accession No. NM_153809, a.a. 1398 – 1649 corresponding to bromodomains 1 and 2 with N-terminal GST-tag, MW = 56.1 kDa, expressed in an E. coli expression system
UniProt
Q8IZX4
Synonym(s):
TAF1 RNA polymerase II, TAT box binding protein (TBP)-associated factor, TAF1L
Assay Conditions:

TAF1L (1398-1649) protein is incubated with biotinylated acetylhistone peptide substrate (Cat. #33000) in TAF1L assay buffer in a 10 µl reaction for 1 hour at RT. AlphaLISA® GSH acceptor beads (PerkinElmer) are added, and the mixture is incubated for 30 minutes at room temperature (RT). AlphaScreen® Streptavidin-conjugated donor beads (PerkinElmer) are then added. After 10 minute incubation at RT, measure Alphacounts.

Formulation:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, and 10% glyercol
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C.
Application(s):
Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Reference(s):

Wang, PJ., et al., Hum. Mol. Genet. 2002 Sep 15; 11(19): 2341-2346.

Application Reference(s):
1. Benzoisoquinolinediones as Potent and Selective Inhibitors of BRPF2 and TAF1/TAF1L Bromodomains (2017)

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Bromodomain
Product Type:
Recombinant Protein
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