SYK, Full length, GST-tag

Catalog #: 40485
Size: 10 µg
$345
Species: Human
Host Species: Sf9 insect cells
MW: 100 kDa
Genbank #: NM_003177
Tag(s): N-terminal GST-tag
a.a: full length
Description:
Recombinant full-length human SYK (GenBank accession #NM_003177) with an N-terminal GST-tag was expressed in Sf9 insect cells via a baculovirus expression system. MW = 10 kDa.
UniProt
P43405
Synonym(s):
SYK, Spleen Tyrosine Kinase SYK
Specific Activity:
95 pmol/min/µg
Assay Conditions:
SYK was incubated with a substrate (Tyr peptide 2) for 1 hour at RT in 1X kinase buffer supplemented with ATP. Developer solution was added to reaction and reaction was stopped after 1 hour of incubation at RT.
Formulation:
45 mM Tris-HCl, pH 8, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione,3 mM DTT and 10% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Takano, T. et al. Antioxid Redox Signal. 2002 Jun,4(3):533-41.
2. Navara, C S. Curr Pharm Des. 2004,10(15):1739-44.

Application Reference(s):
1. Reconstruction and signal propagation analysis of the Syk signaling network in breast cancer cells (2017)

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Cytoplasmic Tyr
Product Type:
Recombinant Protein
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Product Datasheets

40485_Lot100310.pdf