SYK, Full length, GST-tag Recombinant

Catalog #

40485

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant full-length human SYK also known as Spleen Associated Tyrosine Kinase and Tyrosine-Protein Kinase SYK, GenBank Accession No. NM_003177, a.a. full length with N-terminal GST-tag, expressed in a Sf9 expression system. MW = 100 kDa.

This product has been cited 4 times.

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Synonyms

SYK, Spleen Tyrosine Kinase SYK

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Product Data Gallery

4-20 % SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations4

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥50%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione,3 mM DTT and 10% glycerol.

100 kDa

Amino Acids

full length

Specific Activity

108 pmol/min/µg

Genbank #

NM_003177

UniProt #

P43405

Tag(s)

N-terminal GST-tag

References

1. Takano, T. et al. Antioxid Redox Signal. 2002 Jun,4(3):533-41.
2. Navara, C S. Curr Pharm Des. 2004,10(15):1739-44.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 0.2 mM DTT using 0.2 mg/ml Poly(Glu:Tyr) substrate and 20 uM ATP. Reaction was done at 30°C for 35 min. Amount of ATP transferred was calculated using Kinase-Glo reagent from Promega.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Cytoplasmic Tyr

Regulatory Status

Research Use Only

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Datasheets/Protocols

40485 Lot 100310.pdf

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SYK, Full length, GST-tag Recombinant

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