SRE Reporter Kit (MAPK/ERK Signaling Pathway )

Catalog #: 60511
Size: 500 reactions
$525
Background:
The MAPK/ERK signaling pathway is a major participant in the regulation of cell growth and differentiation. It can be activated by various extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, MEK1/2 phosphorylate and activate ERK1/2. The activated ERK translocates to the nucleus where it phosphorylates and activates transcription factors. The TCFs (Ternary Complex Factors), including Elk1, are among the best-characterized transcription factor substrates of ERK. When phosphorylated by ERK, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes.
Description:
The SRE Reporter Kit is designed for monitoring the activity of the ERK signaling pathway and the transcriptional activity of SRF in cultured cells. The kit contains a transfection-ready SRE luciferase reporter vector, which is an ERK pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized SRE responsive elements located upstream of a minimal promoter. The SRE reporter is premixed with a constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity.
Synonym(s):
SRE reporter, MAPK reporter, ERK reporter
Supplied As:
Reporter: SRE luciferase reporter vector + constitutively-expressing Renilla luciferase vector
Negative Control Reporter: Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector
Concentration:

Both the Reporter and Negative Control Reporter are provided at 55 ng DNA/µl (500 µl each)

Format:
assay kit
Instructions for use:
See data sheet for detailed transfection and assay protocols.
Storage / Stability:

Stable at least 12 months from date of receipt, when stored as directed (-20°C)

Application(s):
• Monitor MAPK/ERK signaling pathway activity and SRF-mediated activity.
• Screen for activators or inhibitors of the MAPK/ERK signaling pathway.
• Study effects of RNAi or gene overexpression on the activity of the MAPK/ERK pathway.
Reference(s):
1. Wong, K.K. (2009) Recent developments in anti-cancer agents targeting the Ras/Raf/ MEK/ERK pathway. Recent Pat Anticancer Drug Discov. 4(1):28-35.
2. Treisman, R. (1992) The serum response element. Trends Biochem Sci. 17(10): 423-426.
Notes:
The vectors are suitable for transient transfection. They are NOT suitable for transformation and amplification in bacteria.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Cell Signaling Pathway
Product Type:
Reporter Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

60511.pdf