Sphingosine kinase 2, His-tag

Catalog #: 40611
Size: 20 µg
$385
Species: Human
Host Species: Sf9 insect cells
MW: 69.5 kDa
Genbank #: AF245447
Tag(s): N-terminal His-tag
a.a: full length
Description:
Human Sphingosine kinase 2 (GenBank Accession No. AF245447), full length with N-terminal His-tag, MW=69.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Synonym(s):
SphK2, sphingosine kinase 2, SPK2
Specific Activity:
20 U/µg
Unit Definition:
A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P per minute at 37°C.
Assay Conditions:
Sphingosine kinase activity was determined using D-erythro-sphingosine and ATP as substrates. Assay condition: 50 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, 3 µM Na orthovanadate, 0.5 mM ATP, 4 µM D-erythrosphingosine, and 0.75 μg/ml sphingosine kinase 2 at 37°C for 30 min. ATP reduction is detected using Kinase-Glo® Luminescent Kinase Assay Platform (Promega Corporation, Madison, WI).
Formulation:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 20% glycerol, and 3 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Zemann, B., et al., Blood.107(4):1454-8 (2006)
2. Maceyka, M., et al., J Biol Chem. 280(44):37118-29 (2005)

Application Reference:
1. Synthesis and biological evaluation of carbocyclic analogues of pachastrissamine (2015)
2. Regulation of the hepatitis C virus RNA replicase by endogenous lipid peroxidation (2014)

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Lipids
Product Type:
Recombinant Protein
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Product Datasheets

40611_Lot100219.pdf