Sphingosine kinase 2, His-tag Recombinant

Catalog #

40611

$415 *

Size: 20 µg

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Description

Recombinant human Sphingosine Kinase 2 (SPHK2) variant 4, full length, encompassing amino acids 1-6549(end). This construct contains an N-terminal His-tag (6xHis). The recombinant protein was affinity purified.

This product has been cited 3 times.

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Synonyms

SPHK2, SPK2, SK2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

SPHK2 Activity

Product Info

Storage and Usage

Citations3

Species

Human

Construct

Sphingosine Kinase 2 (His-1-618(end))

Host Species/Expression System

Sf9

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, variable amounts of Imidazole, and 20% Glycerol

70 kDa

Amino Acids

1-618(end)

Specific Activity

20 pmol/min/µg

Unit Definition

A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P per minute at 37°C.

Genbank #

NM_001204160

UniProt #

Q9NRA0

Tag(s)

N-terminal His-tag

References

1. Zemann, B., et al., Blood.107(4):1454-8 (2006)
2. Maceyka, M., et al., J Biol Chem. 280(44):37118-29 (2005)

Storage/Stability

At least 6 months at –80°C

Assay Conditions

The activity of SPHK2 was measured with increasing amounts of protein according to the protocol described in SPHK1 Assay Kit (BPS Bioscience #78026).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Lipids

Regulatory Status

Research Use Only

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Datasheets/Protocols

40611 Lot 151216-B

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Sphingosine kinase 2, His-tag Recombinant

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