Sortase A, Heptamutant, His-Tag Recombinant
Catalog #
71048
$470
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Description
Staphylococcus aureus sortase A Heptamutant (P94R, D160N, D165A, Y187L, E189R, K190E, K196T), a.a 26-206(end) GenBank Accession No. AF162687 with N-terminal His tag, MW = 22 kDa, expressed in an E. coli expression system. This protein is calcium dependent.
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Synonyms
SrtA, sortase A, heptamutant
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Product Data Gallery
4-20% SDS-PAGE Coomassie Staining
Product Info
Storage and Usage
Citations
Species
S. aureus
Host Species/Expression System
E. coli
Purity
≥90%
Format
Aqueous buffer solution
Formulation
40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 400 mM Imidazole and 20% glycerol
MW
22 kDa
Amino Acids
26-206(end)
Genbank #
AF162687
Background
Staphylococcal Sortase A is a bacterial transpeptidase that covalently attaches proteins to the bacterial cell wall by cleaving between threonine and glycine at an LPXTG recognition motif and catalyzes the formation of an amide bond between the carboxyl-group of threonine and the amino-group of the cell-wall peptidoglycan. This chemistry has been exploited as a molecular "stapler" to site-specifically link proteins with the C-terminal LPETGX motif to other proteins or molecules possessing a glycine or aminomethylene motif. This reaction is applicable to proteins in solution and on the living cell surface.
References
1. Chen, I. et al., Proc Natl. Acad. Sci. (USA). 2011; 108(28):11399-11404.
2. Kappel, K., et al., Protein Sci. 2012; 21(12):1858-71.
3. Chen, L., et al., Sci. Rep. 2016; (6):31899.
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