The SMYD4 Chemiluminescent Assay Kit is designed to measure SMYD4 activity for screening and profiling applications. The SMYD4 Chemiluminescent Assay Kit comes in a convenient format, with 96-well plate precoated with a specific substrate, the antibody against methylated lysine residue of Histone H3, a secondary HRP-labeled antibody, Sadenosylmethionine, methyltransferase assay buffer, and purified SMYD4 enzyme for 96 enzyme reactions. The key to the SMYD4 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
6 months from date of receipt when stored as directed.
Instructions for Use
See product datasheet for detailed protocol.
Applications
Useful for studying enzyme kinetics and HTS applications.
Shipping Temperature
dry ice
Notes
MATERIALS REQUIRED BUT NOT SUPPLIED: TBST buffer (1x Tris-buffered saline (TBS), pH 8.0, containing 0.05% Tween-20) Luminometer or microplate reader capable of reading chemiluminescence Adjustable micropipettor and sterile tips Rotating or rocker platform
Contraindications
DMSO >1%, strong acids or bases, ionic detergents, high salt
Warnings
Avoid freeze/thaw cycles.
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.