SMYD2 (KMT3C) Chemiluminescent Assay Kit
The SMYD2 Chemiluminescent Assay Kit is designed to measure SMYD2 activity for screening and profiling applications. The key to the SMYD2 Chemiluminescent Assay Kit is a highly specific antibody that recognizes a methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and the SMYD2 methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
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TBST buffer (1x Tris-buffered saline (TBS), pH 8.0, containing 0.05% Tween-20)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform
Catalog |
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80 µg |
-80°C |
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2 x 250 µl |
-80°C |
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52140P2 |
Primary antibody 16-2 |
12.5 µl |
-80°C |
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10 µl |
-80°C |
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3 ml |
-20°C |
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52100 |
Blocking buffer 4 |
50 ml |
+4°C |
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6 ml |
RT |
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6 ml |
RT |
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White microplate precoated with histone substrate |
1 |
+4°C |