SMURF2, FLAG-tag Recombinant

Catalog #

80403

$400 *

Size: 20 µg

Qty

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Description

Human SMURF2 (GenBank Accession No. NM_022739), a.a. 130-end, with N-terminal FLAG-tag, MW= 72 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

SMAD specific E3 ubiquitin protein ligase 2, SMURF2

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Product Data Gallery

SMURF2 Specific Activity

10% SDS-PAGE Coomassie Staining

SMURF2 inhibition curve using a TR-FRET assay (#78304). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 ?g/ml FLAG peptide, 10% glycerol, and 3 mM DTT.

72 kDa

Amino Acids

130–end

Genbank #

NM_022739

UniProt #

Q9HAU4

Tag(s)

N-terminal FLAG-tag

References

1. Narimatsu M. et al., Cell 137 (2), 295-307 (2009)
2. Fukunaga E. et al., J. Biol. Chem. 283 (51), 35660-35667 (2008).

Storage/Stability

>6 months at -80°C.

Assay Conditions

50 µl reaction mix (4 mM ATP, 5 mM MgCl₂, 1 mM DTT, E1 (0.5 µg), E2 (2.8 µg), Biotin-Ubiquitin (0.5 µg) and SMURF2 (0-2 µg) in 50 mM Tris pH 7.4) incubate for 30 min. at 37°C, then add to streptavidin coated plates and incubate for 30 min at 37°C. Add antibody against SMURF2, incubate 1 hr. Finally, incubate 1 hr. with the Europium-labeled secondary antibody. Time resolved fluorescence measured at room temperature (λexc=330 nm, λem=620 nm).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ubiquitin E3 ligase

Regulatory Status

Research Use Only

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SMURF2, FLAG-tag Recombinant

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