SMARCA2 TR-FRET Assay Kit

Catalog #

40342

$550 *

Size: 384 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The SMARCA2 TR-FRET Assay Kit is designed to measure the inhibition of SMARCA2 (BRM) binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, SMARCA2, substrate, and an inhibitor is incubated for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence reader.

Need us to run inhibitor screens or profile your compounds against SMARCA2? Check out our Bromodomain Screening Services.

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Synonyms

SMARCA2, BRM, SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily A, Member 2, AT-Rich Interaction Domain 1B, ARID1B, HTRF

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

TR-FRET

Format

Catalog #	Component	Amount	Storage
31141	SMARCA2	10 µg	-80°C	Avoid freeze/thaw cycles!
33003	Bromodomain Ligand 2	50 µl	-80°C
	Tb-labeled donor	2 x 10 µl	-20°C
	Dye-labeled acceptor	2 x 10 µl	-20°C
33012	3x BRD TR-FRET Assay Buffer 1	4 ml	-20°C
79969	White 384-well microplate	1	Room temp.

*The concentrations of protein are lot-specific and will be indicated on the tubes containing the protein.

UniProt #

P51531

References

Schiaffino-Ortega, S., et al., 2014. J. Hematol. Oncol. 7:81.

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol

Shipping Temperature

-80°C (dry ice)

Notes

The final concentration of DMSO should not exceed 0.5%.

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Bromodomain

Regulatory Status

Research Use Only