SLC5A5 – HEK293 Recombinant Cell line

Catalog #

90333

$9,400 *

Size: 2 vials

Qty

Bulk/Custom

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Description

Stable recombinant HEK293 cell line expressing human SLC5A5 (UniProt # Q92911) with a C-terminal FLAG- tag. SLC5A5 is a sodium-dependent iodide transporter and is a member of the sodium glucose co-transporter family. The expression of SLC5A5 is confirmed by Western blotting. This cell line can be used to evaluate agents that may affect iodide transport.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Time dependent uptake of Iodide by SLC5A5 - HEK293 cells

ITB5 dependent inhibition of Iodide uptake by SLC5A5 - HEK293 cells

Concentration dependent uptake of Iodide by SLC5A5 - HEK293 cells

Western Blot of the SLC5A5 expressing cells

Product Info

Storage and Usage

Citations

Host Cell Line

HEK293 cells

Format

Each vial contains ~1.5 X 10⁶ cells in 1 ml of 10% DMSO.

UniProt #

Q92911

Background

Iodine transport is essential for the production of thyroid hormone (T4). Sodium Iodide Symporter SLC5A5 (NIS) is a sodium-dependent transporter of iodide into thyroid follicular cells and other peripheral tissues to support T4 synthesis [1]. Lack of iodide transport can result in hypothyroidism and can manifest itself in several disease states like goiter formation and weight gain. SLC5A5 also plays an important role in hyperthyroidism (an excessive production of T4). Expression and activity of SCL5A5 is altered in several forms of cancer. Inhibitors of SLC5A5 have potential therapeutic benefit for the regulation of iodine overload induced by amiodarone or other thyromimetics that can result in hyperthyroidism [2].

References

1. Dohán et al., (2003) The sodium/iodide Symporter (NIS): characterization, regulation, and medical significance. Endocrine Reviews 24(1):48-77.

2. Lindenthal et al., (2009) Characterization of NIS inhibitors. Journal of Endocrinology 200: 357–365.

3. Waltz et al., (2010) A nonradioactive iodide uptake assay for sodium iodide symporter function. Analytical Biochemistry 396(1): 91-5.

Storage/Stability

Immediately upon receipt, store in liquid nitrogen.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 1 (BPS Cat. #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin

Growth Medium 1B (BPS Cat. #79531): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 400 µg/ml of Geneticin

Instructions for Use

See cell line data sheet for detailed culturing and assay protocol.

Applications

• Drug compound screening

• Functional assays

Shipping Temperature

-80°C (dry ice)

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ion Channel

Regulatory Status

Research Use Only