Sirtuin 3, GST-tag

Catalog #: 50014
Size: 100 µg
$425
Species: Human
Host Species: E. coli
MW: 59 kDa
Genbank #: NM_012239
Tag(s): N-terminal GST-tag
a.a: 102–399
Description:
Human Sirtuin 3 (GenBank Accession No. NM_012239), a.a. 102-399 with N-terminal GST-tag, MW= 59 kDa, expressed in an E. coli expression system.
UniProt
Q9NTG7
Synonym(s):
Sirtuin3, SIRT3
Specific Activity:
3.7 pmole/min/µg
Assay Conditions:
25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM HDAC substrate 1 (Catalog #50032), 500 µM NAD+ and Sirtuin 3 (31 - 2000 ng). Incubation condition: 30 min at 37°C followed by treatment with 50 µl of SIRT developer (Catalog # 50089) for 15 min at RT. Fluorescence intensity is measured at ex360/em460.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Scher, M.B. et al., Genes Dev. 21 (8), 920-928 (2007)
2. Bellizzi, D. et al., Genomics 89 (1), 143-150 (2007)
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Deacetylase
Product Type:
Recombinant Protein
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Product Datasheets

50014_Lot_181005.pdf