SIRP-γ (CD172g), Fc fusion, Biotin-labeled (Human) HiP™ Recombinant
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Catalog #
71236
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Description
Signal regulatory proteingamma (SIRP-γ), also known as signal regulatory protein beta 2, and CD172g, GenBank Accession No. NP_018556, a.a. 29-360, fused at the C-terminus to the Fc portion of human IgG1 and Cterminal Avitag™, MW 67.6 kDa, expressed in a HEK293 expression system and enzymatically biotinylated using Avitag™ technology. Note: SIRP-γ is heavily glycosylated, resulting in higher molecular weight. Biotinylation was confirmed to be >90%.
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Synonyms
Signal regulatory protein-gamma, signal regulatory protein beta 2, and CD172g
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Product Data Gallery
4-20% SDS-PAGE Coomassie Staining
Gel Filtration Curve
CD47: SIRP-γ[Biotinylated] inhibition curve using a chemiluminescent assay (#72059). Representative data only; data may vary by lot.
8 mM Phosphate, pH 7.4,
110 mM NaCl, 2.2 mM KCl, and 20%
glycerol
MW
67.6 kDa + glycans
Amino Acids
29-360
Genbank #
NP_018556
UniProt #
Q9P1W8
Tag(s)
C-terminal Fc fusion, C-terminal Avitag™
Label
This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation confirmed to be ≥90%.
For more information on enzymatic biotinylation, please see our Tech Note.
References
1. Barclay, AN and Brown, MH., Nature Rev. Immunol. 2006; 6: 457-464.
Storage/Stability
At least 6 months at -80°C
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Applications
Useful for studying protein binding and screening small molecules.