Monkey Signal regulatory protein-alpha (SIRP-α), also known as Tyrosine-protein phosphatase non-receptor type substrate 1, CD172a, Bit, and MyD-1, GenBank Accession No. NM_001284750, a.a. 31-370, with C-terminal Avi-His-tag, expressed in a HEK293 expression system. MW=42 kDa. The protein is enzymatically biotinylated using AviTag™ technology. This protein is heavily glycosylated, resulting in apparent higher molecular weight.
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Synonyms
Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1, CD172a, Bit, and MyD-1
8mM phosphate, 110mM NaCl, 2.2mM KCl, pH7.4, and 20% glycerol.
MW
42 kDa
Amino Acids
31-370
Genbank #
NM_001284750
Tag(s)
C-Terminal Avi-His-Tag, Biotin-Labeled
Label
This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation confirmed to be ≥90%.
For more information on enzymatic biotinylation, please see our Tech Note.
References
1. Hatherley, D., et al., Mol. Cell, 2008, 31:266-77
2. Baba, N., et al., J. Exp. Med., 2013, 210(6):1251-63
Storage/Stability
At least 6 months at –80°C.
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.