SHP-2 (PTPN11), GST-tag Recombinant

Catalog #

30022

$325 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Protein tyrosine phosphatase SHP-2, also known as PTPN11 (GenBank accession number NM_002834), a.a. 224-529 (catalytic domain) with N-terminal GST-tag, MW=62 kDa, expressed in an E. coli expression system.

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Synonyms

SHP2, CFC, NS1, BPTP3, PTP2C, PTP-1D, SH-PTP2, SH-PTP3, MGC14433, SHP

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.

62 kDa

Amino Acids

224–529

Specific Activity

>1,000 pmol/min/µg

Genbank #

NM_002834

UniProt #

Q06124

Tag(s)

N-terminal GST-tag

Background

Mammalian PTPases can be subdivided into 1 of 2 broad categories: transmembrane receptor PTPases and intracellular PTPases. PTPN11 is one of the 2 closely related mammalian intracellular PTPases whose sequences encode 2 tandem SRC homology 2 (SH2) domains that are located at the amino-terminal side of a single PTPase catalytic domain. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration.

References

1. B.G. Neel et al. Trends Biochem. Sci. (2003) 28:284.
2. J.V. Ravetch and L.L. Lanier Science (2000) 290:84.
3. S. Yamazaki et al. J. Infect. Dis. (2003) 187:334.

Storage/Stability

>12 months at -80°C.

Assay Conditions

50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50 mM pNPP.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

PTP/Non-rec.

Regulatory Status

Research Use Only

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Datasheets/Protocols

30022_Lot130807A.pdf

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SHP-2 (PTPN11), GST-tag Recombinant

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