SetDB1, His-GST-tags Recombinant

Catalog #

51055

$465 *

Size: 20 µg

Qty

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Description

Human SetDB1 (variant 2), also known as Histone-lysine Nmethyltransferase SETDB1, SET domain bifurcated 1, KMT1E, ERG-associated protein with SET domain (ESET), GenBank Accession No. NM_012432, a.a. 510- 1290(end) expressed in a Baculovirus Sf9 cell expression system. MW = 114 kDa.

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Synonyms

Histone-lysine Nmethyltransferase SETDB1, SET domain bifurcated 1, KMT1E, ERG-associated protein with SET domain (ESET)

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

SetDB1 Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

SetDB1 (His-GST-510-1290(end))

Host Species/Expression System

Sf9

Purity

≥85%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% Glycerol and variable glutathione

114 kDa

Amino Acids

510-1290(end)

Genbank #

NM_012432

UniProt #

Q15047

Tag(s)

N-terminal His-GST-tags

References

1. Dillon, S.C., et al., Genom. Biol. 2005; 6: 227.
2. Schultz, D.C. et al., Genes Dev. 2002; 16: 919-932.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay run according to SetDB1 Chemiluminescent Assay Kit (BPS Bioscience #51056).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only