SETD2 Chemiluminescent Assay Kit

Catalog #
52060
$750 *
Size: 96 reactions
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Description

The SETD2 Direct Activity Chemiluminescent Assay Kit is designed to measure SETD2 activity for screening and profiling applications. The key to the SETD2 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K36 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

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This product has been cited 2 times.

Synonyms
SET Domain Containing 2, HYPB1, HIF-1, KMT3A, KIAA1732, SETD2, PRDM2
Product Info
Storage and Usage
Citations2
Assay Kit Format
Chemiluminescent
Supplied As
Kit comes in a convenient strip format, with a 96-well plate precoated with histone H3 peptide substrate, purified SetD2 and reagents for 100 enzyme reactions.
Format
Catalog
Number

Component 

Amount

Storage
53019 SETD2 human enzyme 10 µg -80°C

Avoid freeze/
thaw cycles!
52120 400 μM S-adenosylmethionine 250 µl -80°C
52140L Primary antibody 12 100 µl -80°C
52131H Secondary HRP-labeled antibody 2 10 µl -80°C
52160 4x HMT assay buffer 1* 3 ml -20°C
79556 Blocking buffer 50 ml +4°C  
  HRP chemiluminescent substrate
(2 components)

6 ml each
+4°C  
  96-well plate precoated with histone
substrate

1 plate

 

+4°C

 
UniProt #
Q9BYW2
Background
SETD2 is a histone methyltransferase that exhibits methylation activity toward histones H3-K36
References

Dillon S.C., et al. 2005. Genome Biology 6:227.