Set7/9 (Sf9), His-GST-tags Recombinant

Catalog #

50019

$320 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Human recombinant SET domain containing lysine methyltransferase 7 (SETD7), or SET7/9 (GenBank Accession No. NM_030648), a.a. 52-266 (end) with N-terminal His-GST-tag, MW = 63 kDa, expressed in a baculovirus infected Sf9 cell expression system.

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Synonyms

Set7/9, SETD7, KIAA1717, KMT7, SET7, SET9

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥62%

Format

Aqueous buffer solution

Formulation

40 mM Tris, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, and 20% glycerol

63 kDa

Amino Acids

52–266 (end)

Genbank #

NM_030648

UniProt #

Q8WTS6

Tag(s)

N-terminal His-GST-tags

Background

SETD7 or SET domain containing lysine methyltransferase 7 is a lysine methyltransferase which can methylates lysine-4 (K4) in histone H3 in vitro and in vivo. Methylation of K4 in histone H3 by SETD7 and methylation of K9 in histone H3 by SUV39H1 were found to have differential effects on subsequent histone acetylation by p300. SETD7 can also methylate p53 at lys372 within the C-terminal regulatory region. Methylated p53 is restricted to the nucleus and the modification positively affects its stability. SETD7 regulates the expression of p53 target genes in a manner dependent on the p53 methylation site.

References

1. Ivanov G.S. et al., Mol. Cell. Biol. 27 (19), 6756-6769 (2007).
2. Couture J.F. et al., Nat. Struct. Mol. Biol. 13 (2), 140-146 (2006).

Application Reference(s):
Selective inhibitors of bacterial t-RNA-(N(1)G37) methyltransferase (TrmD) that demonstrate novel ordering of the lid domain (2013)

Storage/Stability

>6 months at -80°C.

Assay Conditions

50 µl reaction mix (50 mM Tris pH 8.8, 5 mM MgCl₂, 4 mM DTT, 20 µM S-adenosylmethionine, and 0-10 ng SET7/9) add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated K4 residue of histone H3, incubate 1 hr. Then incubate 30 mins with HRP-labeled secondary antibody. Finally, add HRP chemiluminescent substrates and read luminescence.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only