SBE Reporter Kit (TGFβ/SMAD signaling pathway)

Catalog #: 60654
Size: 500 reactions
$525
Description:
The SBE Reporter kit is designed for monitoring the activity of TGFβ/SMAD signaling pathway in the cultured cells. The kit contains transfection-ready SBE luciferase reporter vector, which is a TGFβ pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized SBE responsive element located upstream of a minimal promoter. The SBE reporter is premixed with constitutively-expressing Renilla luciferase vector that serves as internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control. The noninducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.
Synonym(s):
SBE reporter, TGFβ reporter, TGFbeta reporter, SMAD reporter
Supplied As:
Reporter: SBE luciferase reporter vector + constitutively-expressing Renilla luciferase vector
Negative Control Reporter: Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector
Concentration:

Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each)

Format:
assay kit
Instructions for use:
See data sheet for detailed transfection and assay protocols.
Storage / Stability:

Stable at least 12 months from date of receipt, when stored as directed (-20°C)

Application(s):
• Monitor TGFβ signaling pathway activity.
• Screen for activators or inhibitors of the TGFβ/ SMAD signaling pathway.
• Study effects of RNAi or gene overexpression on the activity of the TGFβ pathway.
Reference(s):
Moustakas A et al. (2001) Smad regulation in TGF-beta signal transduction. J.Cell Science. 114(Pt 24): 4359-69.
Notes:
The vectors are suitable for transient transfection. They are NOT suitable for transformation and amplification in bacteria.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Cell Signaling Pathway
Product Type:
Reporter Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

60654_1.pdf