SBE Reporter Kit (TGFβ/SMAD signaling pathway)

Catalog #
60654
$595 *
Size: 500 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The SBE Reporter kit is designed for monitoring the activity of TGFβ/SMAD signaling pathway in the cultured cells. The kit contains transfection-ready SBE luciferase reporter vector, which is a TGFβ pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized SBE responsive element located upstream of a minimal promoter. The SBE reporter is premixed with constitutively-expressing Renilla luciferase vector that serves as internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control. The noninducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.

For assays in more difficult to transfect cell types, we suggest using our lentiviral products. The corresponding lentiviral product for this kit is BPS Bioscience #79806.

This product has been cited 11 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

Synonyms
SBE reporter, TGFβ reporter, TGFbeta reporter, SMAD reporter , Cell based assay kit, cell-based assay, reporter assay kit, TGFb-1, TGFb1
Product Info
Storage and Usage
Citations11
Supplied As
Reporter: SBE luciferase reporter vector + constitutively-expressing Renilla luciferase vector
Negative Control Reporter: Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector
Format
assay kit
Concentration

Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each)

References

Moustakas A et al. (2001) Smad regulation in TGF-beta signal transduction. J.Cell Science. 114(Pt 24): 4359-69.