Renin, His-Tag Recombinant

Catalog #

80200

$455 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Recombinant human renin, encompassing amino acids 67-406(end). This construct contains a C-terminal His-tag (10xHis). This protein was affinity purified.

This product has been cited 2 times.

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Synonyms

Angiotensinogenase, Renin Protein, Renin his tag, Renin protease, renin , renin angiotensin aldosterone system, renin angiotensin aldosterone

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Renin inhibition curve using a fluorogenic assay (#80211). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations2

Species

Human

Host Species/Expression System

HEK293 cells

Purity

≥80%

Supplied As

Aqueous buffer solution.

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 250 mM imidazole

39 kDa

Amino Acids

67–406

Specific Activity

255 pmol/min/μg

Genbank #

NM_000537

UniProt #

P00797

Tag(s)

C-terminal His-tag

Background

Renin, or angiotensinogenase, is an aspartic protease that is synthesized by the kidneys, and it is part of the RAAS (renin-angiotensin-aldosterone system). Renin catalyzes the hydrolysis of angiotensinogen to angiotensin I, which is converted to angiotensin II in the lungs by ACE (angiotensin-converting enzyme), a highly potent vasoconstrictor. Renin is secreted in response to changes in arterial blood pressure, and to sodium decreases in the distal tubules in response to activity of the sympathetic nervous system. Renin inhibitors have been used therapeutically in the treatment of hypertension (high blood pressure).

References

1. Cho, M.J., et al., J Pediatr Endocrinol Metab. 2011; 24(11-12):1085 – 1087
2. Schnermann, J., et al., Kidney Int. 2012 Mar; 81(6): 529 – 538

Storage/Stability

At least 6 months at -80°C

Assay Conditions

Assay was done according to the Renin Assay Kit (BPS Bioscience #80211) with Renin titrated from at various amounts.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Protease

Regulatory Status

Research Use Only