Renin Assay Kit

Catalog #: 80211
Size: 96 reactions
$315
Background:
Renin is a protease that is synthesized by the kidneys. Renin catalyzes the hydrolysis of angiotensinogen to angiotensin I, which is converted to angiotensin II, a highly potent vasoconstrictor. Renin inhibitors have been used therapeutically in the treatment of hypertension (high blood pressure).
Description:
The Renin Assay Kit is designed to measure Renin activity for screening and profiling applications. It comes in a convenient 96-well format, with purified, activated renin, renin substrate, and assay buffer for 100 enzyme reactions. Aliskiren Hydrochloride is also included as a control for renin inhibition. The key to the Renin Assay Kit is the specific, fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for renin reactions. The fluorometric substrate is incubated with a sample containing renin enzyme to produce a fluorophore that can then be measured using a fluorescence reader.
Synonym(s):
Angiotensinogenase
Supplied As:
Supplied in a convenient 96-well format, with purified, activated renin, renin substrate, assay buffer, and inhibitor for 100 enzyme reactions.
Contraindications:
DMSO >1%, strong acids or bases, ionic detergents, high salt
Format:
Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:

At least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Reference(s):
1. Paschalidou, K. et al., Biochem. J. 2004; 382: 1031.
2. Gossas, T., et al., Naunyn Schmiedebergs Arch Pharmacol. 2012; 385(2):219-24.
Notes:
MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Fluorescent microplate reader
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Protease
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

80211.pdf