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Recombinant Nucleosome (E. coli-derived)

Catalog #

52333

$3,130 *

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Description

Recombinant human nucleosome consisting of 147 bp DNA (PCR) and 2 molecules each of histones H2A, encompassing amino acids 2-130(end), H2B encompassing amino acids 2-126(end), H3 encompassing amino acids 2-137(end), and H4 encompassing amino acids 2-103(end). Each histone construct contains an N-terminal His-tag (6xHis). The recombinant complex was affinity purified.

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Product Data Gallery

4-20% Native gel Coomassie Staining

4-20% Native gel Ethidium Bromide staining

4-20% SDS-PAGE gel Ethidium Bromide staining

4-20% SDS-PAGE gel Coomassie staining

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. Coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

20 mM Tris pH 7.5, 1 mM EDTA, 1 mM DTT and 20% glycerol

MW

113.8 kDa

Amino Acids

H2A 2-130(end), H2B 2-126(end), H3 2-137(end), H4 2-103(end)

Genbank #

NM_033445, NM_003528, NM_003532, and NM_003548

UniProt #

Q7L7L0, Q16778, P68431, P62805

References

1. Meagher, R.B., Mussar, K.J., Epigenetics Chromatin 2012 Jul 20;5(1):11.
2. Huang, H., et al., Biosystems 2012 Aug;109(2):214-9.

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