Recombinant Nucleosome (E. coli-derived)

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Catalog #

52038

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Description

Recombinant human nucleosome complex consisting of 147 bp DNA (PCR) and 2 molecules each of histones H2A, encompassing amino acids 2-130(end), H2B encompassing amino acids 2-126(end), H3 encompassing amino acids 2-136(end), and H4 encompassing amino acids 2-103(end). Each histone construct contains an N-terminal His-tag (6xHis). The recombinant complex was affinity purified.

This product has been cited 4 times.

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Synonyms

Histone H2A type 3, H2A-clustered histone 25, H2AC25, H2AW, HIST3H2A, Histone H2B type 2-E, H2B-clustered histone 21, Histone H2B-GL105, Histone H2B.q (H2B/q), H2BC21, H2BFQ, HIST2H2BE, Histone H3.1, Histone H4, H4C1, H4/A, H4FA, HIST1H4A

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

4-20% SDS-PAGE EtBr Staining

4-20% Native-PAGE Coomassie Staining

4-20% Native-PAGE EtBr Staining

Product Info

Storage and Usage

Citations4

Species

Human

Construct

Histone H2A (His-2-130(end) / Histone H2B (His-2-126(end)) / Histone H3 (His-2- 136(end)) / Histone H4 (His-2-103(end))

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

20 mM Tris, pH 7.5, 1 mM EDTA, 1 mM DTT, and 20% glycerol

Histone H2A: 15 kDa; Histone H2B: 15 kDa; Histone H3: 16 kDa; Histone H4: 12 kDa

Amino Acids

Histone H2A: 2-130(end); Histone H2B: 2-126(end); Histone H3: 2-136(end); Histone H4: 2-103(end)

Genbank #

Histone H2A: NM_033445; Histone H2B: NM_003528; Histone H3: NM_003532; and Histone 4: NM_003548

UniProt #

Histone H2A: Q7L7L0; Histone H2B: Q16778; Histone H3: P68431; Histone H4: P62805

Tag(s)

N-terminal His-tag

Background

Nucleosomes, formed of histones, are basic units of chromatin and play a central role in DNA compacting and organization within the nucleus of eukaryotic cells. Nucleosomes positioning along the DNA allows access to transcription factors and regulatory proteins to specific regions, influencing whether a gene is turned on or off. Enzymes involved in transcriptional regulation, in DNA replication, or in DNA damage responses can modify histones through phosphorylation, acetylation, methylation, and more. Therefore, nucleosomes can be used as substrate for these enzymes, among other uses.

References

1. Meagher, R.B., Mussar, K.J., Epigenetics Chromatin 2012 Jul 20;5(1):11.
2. Huang, H., et al., Biosystems 2012 Aug;109(2):214-9.

Storage/Stability

At least 6 months at –80°C.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Substrate for histone methyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases for drug discovery and HTS applications.

Shipping Temperature

-80°C

Notes

The concentration of this product is determined by Bradford and represents only the protein concentration.

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only