JNK Signaling Pathway AP1 Reporter - HEK293 Cell line
Catalog #: 60405

Description
The stress-activated protein kinase/c-jun N-terminal kinase (SAPK/JNK) family of
proteins includes mitogen-activated protein kinases (MAPKs) that are activated by stress,
inflammatory cytokines, mitogens, oncogenes, and inducers of cell differentiation and
morphogenesis. Upon activation of the SAPK/JNK pathway, MAP Kinase Kinases
phosphorylate and activate JNKs. The activated JNKs translocate to the nucleus where
they phosphorylate and activate transcription factors such as c-Jun. cJun then binds to
the activator protein-1 (AP1) response element and induces AP1 transcription.
The AP1 Reporter – HEK293 cell line contains a firefly luciferase gene under the control
of AP1-responsive elements that are stably integrated into HEK293 cells. This cell line is
validated for its response to stimulation by Phorbol 12-Myristate 13-Acetate (PMA) and
to treatment with inhibitors of the JNK signaling pathway.

Applications
• Monitor the JNK signaling pathway activity and AP1-mediated activity.
• Screen for activators or inhibitors of the JNK signaling pathway.

Format
Each vial contains ~1.5 X 10^6 cells in 1 ml of 10% DMSO.

References
1. Zhou H. et. al. (2005) Frequency and distribution of AP-1 sites in the human
genome. DNA Research. 11: 139-150.
2. Gaillard P. et.al. (2005) Design and synthesis of the first generation of novel
potent, selective, and in vivo active (benzothiazol-2-yl)acetonitrile inhibitors of the
c-Jun N-terminal kinase. J Med Chem. 48(14):4596-4607.

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