JAK/STAT Signaling Pathway
ISRE Reporter – HEK293 Cell Line Call Us for a Custom Quote
Catalog #: 60510
The ISRE Reporter – HEK293 Cell Line is designed for monitoring the activity of the
JAK/STAT signaling pathway. The JAK (Janus kinase) /STAT (Signal Transducer and
Activator of Transcription) pathway is activated by various cytokines and growth factors
and plays a critical role in cell growth, hematopoiesis, and immune response. In
mammals, there are four JAKs (JAK1, JAK2, JAK3 and TYK2) and seven STAT proteins.
Binding of Interferon alpha (IFNα) to its receptor leads to the activation of JAK1 and
TYK2, which in turn phosphorylate and activate STAT1 and STAT2. The phosphorylated
STAT1 and 2 form a heterodimer and bind to IRF9/p48, forming a protein complex
known as ISGF3. This complex translocates to the nucleus and binds to the ISRE
(Interferon Stimulated Response Element) in the promoter region thereby promoting
transcription of interferon-inducible genes.
The ISRE Reporter – HEK293 Cell Line contains the firefly luciferase gene under the
control of ISRE stably integrated into HEK293 cells. This cell line is validated for the
response to stimulation with interferon Alpha A and to treatment with JAK inhibitor.
• Monitor IFNα-induced activity and the JAK/STAT pathway activity.
• Screen for activators or inhibitors of the JAK/STAT pathway.
Each vial contains ~1.5 X 106 cells in 1 ml of 10% DMSO.
Immediately upon receipt, store in liquid nitrogen.
1. Hebenstreit D et al. (2005) JAK/STAT-dependent gene regulation by cytokines.
Drug News Perspect 18 (4): 243–249.
2. Pedranzini L et al. (2006) Pyridone 6, a pan-Janus-activated kinase inhibitor,
induces growth inhibition of multiple myeloma cells. Cancer Res. 66 (19):9714-