Rat VEGF-A Recombinant

Catalog #

91008

$220 *

Size: 10 µg

Qty

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Description

Recombinant rat vascular endothelial growth factor A (VEGF-A) expressed in E. coli cells. Rat recombinant VEGF-A contains 165 amino acids and has a molecular weight of 38.7 kDa. VEGF is an important growth factor involved in both vasculogenesis and angiogenesis.

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Synonyms

vascular endothelial growth factor A, VEGF-A, vascular permeability factor, VPF

Product Info

Storage and Usage

Citations

Species

Rat

Host Species/Expression System

E. coli

Purity

>97%, as determined by SDS-PAGE and HPLC

Format

Lyophilized powder

Formulation

Recombinant rat VEGF lyophilized from 10 mM Na Phosphate, pH 7.5.

38.7 kDa

Biological Activity

Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 4.0 – 8.0 ng/ml.

Genbank #

AF080594.1

References

1. Caires, K.C., et al. Reproduction. 2009; 138: 667-677.
2. Linderholm, B.K., et al. Ann. Onc. 2009; 20: 1639-1646.

Storage/Stability

The lyophilized protein is stable at room temperature for 3 weeks and for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at 2 - 8° C for up to one week, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Reconstitution

It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Shipping Temperature

room temp.

Scientific Category

Cytokines

Regulatory Status

Research Use Only